Exposure of cardiac preparations to solutions containing more than 5 mM-halothane irreversibly damaged the ability of the SR to load Ca2+. The presence of nucleotide (11 mM-AMP) did not affect the halothane-induced Ca2+ release when the Ca2+ pump was inactive. RRed (10 microM) completely blocked the Ca2+ release induced by both concentrations of halothane. This effect was more marked at 1.89 mM than at 0.47 mM. Halothane (0.47 and 1.89 mM) reversibly increased the rate of Ca2+ release from the SR regardless of whether or not the SR Ca2+ pump was active. This indicated that the Ca2+ pump can efficiently recycle Ca2+ lost from the SR under these conditions. The amount of Ca2+ loaded by the 10 min saponin-treated trabeculae was maintained constant for at least 3 min when the preparations were transferred to low solutions (0.1 mM-EGTA pCa greater than 7.5) containing ATP. Exposure of the saponin-treated cardiac preparation to 11 mM-AMP, when the SR Ca2+ pump was not active, enhanced Ca2+ release from the SR by a mechanism which was blocked by 10 microM-RRed. Exposure of rat cardiac trabeculae to a solution containing 50 micrograms/ml saponin for 10 min or longer caused an SR Ca2+ efflux which was not blocked by Ruthenium Red (RRed) and did not require the presence of nucleotides. A method has been developed to study Ca2+ fluxes across the sarcoplasmic reticulum (SR) of chemically (saponin) skinned myocardium without interference from the SR Ca2+ pump. wow just wow Samantha, Netgalley 'There was so much emotion in this story that I finished it with tears rolling down my face' Angela, reviewer 'Amazing.Incredibly powerful and emotional too' Sally, Netgalley 'It's excellent.like all the best stories, simply about people and how they behave' Anne, Netgalley 'Wow! A must read for anyone with an interest in WW2' Sarah, reviewer 'A thought-provoking and disturbing story but one which I feel needs to be heard' Lisa C, reviewer 'Utterly captured every ounce of my concentration.I didn't want to put it down' Netgalley reviewer 'A fascinating story of how ordinary lives are irrevocably changed by war' Anabelle H, reviewer 'Addictive.1. Caught on the wrong side of history, how far is Rosa willing to go to survive? The International Bestseller 'I'm actually having trouble putting into words just how much I enjoyed this book and what a heartbreaking read - so many emotions and not enough words. Alone and scared, she faces the SS with nothing but the knowledge every bite might be her last. Her husband is fighting on the front line. Twenty-six-year-old Rosa has lost everything to this war. Ten women to taste his food and protect him from poison. The tide is turning in the war and his enemies circle ever closer. Hitler hides away in the Wolfsshanze - his hidden headquarters. 'Written with intelligence and nuance' The Times 'A disturbing, powerful and beautifully written novel based on shockingly real events' Christy Lefteri, author of The Beekeeper of Aleppo 'A thought-provoking read' My Weekly 'Unputdownable' The Herald 'For anybody who enjoyed Cilka's Journey.Phenomenal, eye-opening and heart-breaking' Yahoo Best Books of November Inspired by the powerful true story of Margot Woelk, this is a heartbreaking and gripping historical novel for fans of The Tattooist of Auschwitz and The Beekeeper of Aleppo East Prussia, 1943.
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